6EKE
crystal structure of a Pholiota squarrosa lectin unliganded
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.229, 67.670, 30.985 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 33.830 - 1.700 |
| R-factor | 0.17298 |
| Rwork | 0.171 |
| R-free | 0.21119 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS (20160617) |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | SHELXCD (2013) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.830 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 | 0.349 |
| Rpim | 0.041 | 0.304 |
| Number of reflections | 12666 | 644 |
| <I/σ(I)> | 14.8 | 3 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 4 | 3.6 |
| CC(1/2) | 0.997 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 292 | 18% butane1,4diol, 300mM zinc acetate, 100 mM imidazole ph 7.0 |
