6EKC

Crystal structure of the BSD2 homolog of Arabidopsis thaliana bound to the octameric assembly of RbcL from Thermosynechococcus elongatus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9700
Spacegroup name	P 1
Unit cell lengths	217.918, 218.592, 310.273
Unit cell angles	69.40, 81.51, 65.50

Refinement procedure

Resolution	50.010 - 2.630
R-factor	0.2375
Rwork	0.235
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zxw
RMSD bond length	0.007
RMSD bond angle	1.175
Data reduction software	XDS (Jun 1, 2017)
Data scaling software	Aimless (0.5.28)
Phasing software	MOLREP (11.4.06)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	198.290	198.290	2.670
High resolution limit [Å]	2.630	14.410	2.630
Rmerge	0.145	0.040	1.215
Rmeas	0.171	0.047	1.424
Rpim	0.090	0.024	0.737
Number of reflections	1427774	8676	70398
<I/σ(I)>	6.2
Completeness [%]	98.5	98.3	98.2
Redundancy	3.6	3.6	3.7
CC(1/2)	0.994	0.999	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	21 % PEG 3350 and 0.12M DL-malic acid pH 7.0