6EKB

Crystal structure of the BSD2 homolog of Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2826
Spacegroup name	P 41 21 2
Unit cell lengths	55.708, 55.708, 48.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.1901
Rwork	0.189
R-free	0.21580
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.814
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.850	47.850	1.950
High resolution limit [Å]	1.910	8.950	1.910
Rmerge	0.138	0.046	4.852
Rmeas	0.140	0.046	4.904
Rpim	0.020	0.007	0.699
Number of reflections	6226	88	379
<I/σ(I)>	24.7
Completeness [%]	99.7	99.8	95
Redundancy	48.6	37.6	47.4
CC(1/2)	1.000	1.000	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20 % PEG 10,000, 8 % ethylene glycol and 0.1 M HEPES-NaOH pH 7.5