6EK9
Cytosolic copper storage protein Csp from Streptomyces lividans: Cu loaded form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.081, 64.138, 66.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.220 - 1.500 |
| R-factor | 0.2071 |
| Rwork | 0.206 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eio |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.056 |
| Data reduction software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.230 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.161 |
| Rpim | 0.045 | 0.097 |
| Number of reflections | 20701 | 990 |
| <I/σ(I)> | 9.7 | 4.5 |
| Completeness [%] | 96.4 | 96.6 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.992 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 15 mg/ml protein. 1.4 M ammonium sulfate, 0.1 M MES pH 6.0 |
