6EK8
YaxB from Yersinia enterocolitica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 111.700, 111.700, 169.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.820 - 4.000 |
| R-factor | 0.32625 |
| Rwork | 0.326 |
| R-free | 0.33881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ek4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.069 | 0.958 |
| Number of reflections | 5633 | |
| <I/σ(I)> | 13 | 2.1 |
| Completeness [%] | 98.7 | 99.2 |
| Redundancy | 5 | |
| CC(1/2) | 0.999 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.2 M Na/K-phosphate |
