6EK2

CRYSTAL STRUCTURE OF HUMAN CD81 LARGE EXTRACELLULAR LOOP IN COMPLEX WITH SINGLE CHAIN FV FRAGMENT 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	52.317, 118.060, 130.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.720 - 2.650
R-factor	0.1822
Rwork	0.179
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.720	2.740
High resolution limit [Å]	2.650	2.650
Number of reflections	17696	141
<I/σ(I)>	8	2
Completeness [%]	72.8	5.9
Redundancy	4.6	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	25% PEG3350, 0.1 M Bis-Tris, 0.2 M ammonium acetate