6EJP
Yersinia YscU C-terminal fragment in complex with a synthetic compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-25 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.87290 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 53.740, 69.650, 242.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.390 - 2.480 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jli |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 2.630 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.121 | 0.877 |
| Number of reflections | 16706 | 2613 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 13.3 | 14 |
| CC(1/2) | 0.990 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.8 M potassium dihydrogen phosphate, 0.8 M disodium hydrogen phosphate, 0.1 M Hepes |
