6EJJ

Structure of a glycosyltransferase / state 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	123
Detector technology	PIXEL
Collection date	2016-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	60.510, 125.780, 71.010
Unit cell angles	90.00, 90.28, 90.00

Refinement procedure

Resolution	29.814 - 2.700
R-factor	0.2082
Rwork	0.206
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eji
RMSD bond length	0.005
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.796
High resolution limit [Å]	2.300	2.700
Rmerge	0.212	1.743
Number of reflections	48793
<I/σ(I)>	5.6	1.69
Completeness [%]	96.3	100
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Ammonium acetate, ADA, PEG3350