6EIG

Crystal structure of N24Q/C128T mutant of Channelrhodopsin 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	64.510, 134.060, 78.470
Unit cell angles	90.00, 90.22, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.2662
Rwork	0.265
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eid
RMSD bond length	0.003
RMSD bond angle	0.432
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.700	2.700
Rmerge	0.183	0.937
Rpim	0.142	0.724
Number of reflections	17600
<I/σ(I)>	3.5	0.8
Completeness [%]	96.0
Redundancy	2.4
CC(1/2)		0.223

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	Sodium-potassium phosphates