6EID

Crystal structure of wild-type Channelrhodopsin 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	62.780, 135.770, 78.120
Unit cell angles	90.00, 92.95, 90.00

Refinement procedure

Resolution	20.000 - 2.390
R-factor	0.2094
Rwork	0.209
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ug9
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.480
High resolution limit [Å]	2.390	2.390
Rpim	0.055	0.523
Number of reflections	26159
<I/σ(I)>	8.3	1
Completeness [%]	100.0
Redundancy	34.6
CC(1/2)		0.182

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	sodium potassium phosphate