6EI0
Cytosolic copper storage protein Csp from Streptomyces lividans: apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.576, 93.576, 213.252 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.040 - 1.340 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lmf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.537 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.040 | 1.370 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.066 | 1.452 |
| Rmeas | 0.068 | 1.510 |
| Rpim | 0.016 | 0.409 |
| Total number of observations | 2259045 | |
| Number of reflections | 123798 | 8933 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 18.2 | 13.3 |
| CC(1/2) | 0.999 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 15 mg/ml protein was mixed with an equal volume of reservoir solution containing 1.4 M ammonium sulfate, 0.1 M HEPES pH 7.0 |
