6EI0

Cytosolic copper storage protein Csp from Streptomyces lividans: apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	93.576, 93.576, 213.252
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	81.040 - 1.340
R-factor	0.171
Rwork	0.169
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lmf
RMSD bond length	0.014
RMSD bond angle	1.537
Data reduction software	DIALS
Data scaling software	Aimless (0.5.23)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.040	1.370
High resolution limit [Å]	1.340	1.340
Rmerge	0.066	1.452
Rmeas	0.068	1.510
Rpim	0.016	0.409
Total number of observations	2259045
Number of reflections	123798	8933
<I/σ(I)>	18.1
Completeness [%]	100.0	99.4
Redundancy	18.2	13.3
CC(1/2)	0.999	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	15 mg/ml protein was mixed with an equal volume of reservoir solution containing 1.4 M ammonium sulfate, 0.1 M HEPES pH 7.0