6EHX

scFv AbVance: increasing our knowledge of antibody structural space to enable faster and better decision making in drug discovery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976
Spacegroup name	P 41 2 2
Unit cell lengths	81.559, 81.559, 116.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.680 - 2.200
R-factor	0.209
Rwork	0.207
R-free	0.24100
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall
Low resolution limit [Å]	116.000
High resolution limit [Å]	2.200
Number of reflections	20644
<I/σ(I)>	35.9
Completeness [%]	100.0
Redundancy	25.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	290	NaCl 1.5M, Na Hepes, pH 7.5