6EHW

scFv AbVance: increasing our knowledge of antibody structural space to enable faster and better decision making in drug discovery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.96
Spacegroup name	P 2 21 21
Unit cell lengths	45.396, 70.033, 78.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.730 - 2.190
R-factor	0.214
Rwork	0.212
R-free	0.24800
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.730
High resolution limit [Å]	2.190
Number of reflections	12599
<I/σ(I)>	5
Completeness [%]	100.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	NaCl, Hepes pH 7.5