6EHE
OmpTdeltaL8 (loop L8 deletion mutant of OmpT), an outer membrane protein of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2017-02-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9686 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 43.480, 128.410, 171.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.271 - 2.312 |
| R-factor | 0.2269 |
| Rwork | 0.225 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ehd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.113 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.840 | |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rpim | 0.034 | 0.510 |
| Number of reflections | 18688 | |
| <I/σ(I)> | 13.1 | 1.5 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 6.9 | 5.1 |
| CC(1/2) | 0.900 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 0.05 magnesium acetate, 0.1 M glycine, 32% PEG 400 |
