6EHD
OmpT (in-vitro folded), an outer membrane protein of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.880, 75.840, 49.670 |
| Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
| Resolution | 49.640 - 1.660 |
| R-factor | 0.18772 |
| Rwork | 0.186 |
| R-free | 0.21397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ehb |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.185 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.640 | |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rpim | 0.022 | 0.610 |
| Number of reflections | 62456 | |
| <I/σ(I)> | 14.5 | 1.1 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 3.4 | 2.5 |
| CC(1/2) | 0.900 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M NaCl, 0.1 M MES, 33% v/v PEG 400, 4% v/v ethylene glycol |
