6EHC

OmpUdeltaN (N-terminus deletion mutant of OmpU), outer membrane protein of Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2017-05-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.920
Spacegroup name	P 63
Unit cell lengths	81.360, 81.360, 198.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.520 - 2.020
R-factor	0.19533
Rwork	0.193
R-free	0.24761
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ehb
RMSD bond length	0.017
RMSD bond angle	1.652
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.520
High resolution limit [Å]	2.020	2.020
Number of reflections	48646
<I/σ(I)>	12.3	1.3
Completeness [%]	100.0	100
Redundancy	11.5	11.4
CC(1/2)	0.900	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	33% PEG 300, 0.1 M NaCl, 0.05 M bicine