6EHC
OmpUdeltaN (N-terminus deletion mutant of OmpU), outer membrane protein of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2017-05-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 63 |
| Unit cell lengths | 81.360, 81.360, 198.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.520 - 2.020 |
| R-factor | 0.19533 |
| Rwork | 0.193 |
| R-free | 0.24761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ehb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.652 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.520 | |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 48646 | |
| <I/σ(I)> | 12.3 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 11.4 |
| CC(1/2) | 0.900 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 33% PEG 300, 0.1 M NaCl, 0.05 M bicine |
