6EH9
HA1.7 Human T-Cell Receptor specific for Influenza virus epitope PKYVKQNTLKLAT presented by Human Leukocyte Antigen HLA-DR0101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.460, 49.960, 72.750 |
| Unit cell angles | 90.00, 93.08, 90.00 |
Refinement procedure
| Resolution | 48.870 - 2.490 |
| R-factor | 0.23539 |
| Rwork | 0.232 |
| R-free | 0.29651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gkz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.645 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.870 | 2.550 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.520 | |
| Rmeas | 0.703 | |
| Rpim | 0.353 | |
| Number of reflections | 17434 | |
| <I/σ(I)> | 17.5 | 2.3 |
| Completeness [%] | 98.5 | 99.2 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 15% PEG 4K, 15% Glycerol, 100mM MES pH 7.0 |
