6EH7

F11 Human T-Cell Receptor specific for influenza virus haaemagglutinin epitope PKYVKQNTLKLAT carried by Human Leukocyte Antigen HLA-DR0101

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	.92
Spacegroup name	P 21 21 2
Unit cell lengths	85.420, 114.480, 50.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.300 - 1.890
R-factor	0.18763
Rwork	0.185
R-free	0.23211
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gkz
RMSD bond length	0.019
RMSD bond angle	1.926
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	1.940
High resolution limit [Å]	1.890	1.890
Rmerge		0.706
Rmeas		0.765
Number of reflections	37906
<I/σ(I)>	22.3	2.8
Completeness [%]	98.7	98.7
Redundancy	6.5	6.8
CC(1/2)		0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	25% PEG 1.5K, 100mM SPG buffer pH 6.0