6EG3

Crystal structure of human BRM in complex with compound 15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	136.470, 136.470, 121.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.500 - 2.840
R-factor	0.2305
Rwork	0.229
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fqa 5hzr
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	136.470	2.850
High resolution limit [Å]	2.840	2.840
Number of reflections	27552
<I/σ(I)>	18
Completeness [%]	100.0
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	100mM Tris pH7, 14% Ethanol