6EFF
NCTC10712
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 39.786, 48.268, 99.823 |
Unit cell angles | 101.84, 91.39, 89.90 |
Refinement procedure
Resolution | 47.241 - 1.600 |
R-factor | 0.1817 |
Rwork | 0.180 |
R-free | 0.20780 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.924 |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 96441 | |
<I/σ(I)> | 22.9 | |
Completeness [%] | 92.4 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 7.5, 32% w/v PEG 4000 |