6EFB

GspB Siglec domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	298
Detector technology	CCD
Collection date	2017-10-17
Detector	Bruker Platinum 135
Wavelength(s)	1.542
Spacegroup name	P 1 21 1
Unit cell lengths	24.270, 62.580, 62.930
Unit cell angles	90.00, 98.60, 90.00

Refinement procedure

Resolution	23.606 - 1.900
R-factor	0.1708
Rwork	0.170
R-free	0.18550
RMSD bond length	0.006
RMSD bond angle	0.740
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	14767
<I/σ(I)>	9.3
Completeness [%]	97.3
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M ammonim sulfate, 25% PEG 4000, 15% ethanol. 0.1 M Bis-tris pH 7.0