6EF7

GspB Siglec domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	33.920, 46.210, 72.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.040 - 1.030
R-factor	0.1261
Rwork	0.125
R-free	0.14130
RMSD bond length	0.008
RMSD bond angle	1.010
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.060
High resolution limit [Å]	1.030	1.030
Number of reflections	54763
<I/σ(I)>	25.5
Completeness [%]	95.4
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Ammonium Sulfate, 25% PEG 3350