6EF5
14-3-3 with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95365 |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.691, 94.691, 234.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 82.000 - 2.440 |
| R-factor | 0.1947 |
| Rwork | 0.192 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjb |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.797 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 82.000 | 82.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 9.130 | 2.440 |
| Rmerge | 0.222 | 0.060 | 1.036 |
| Rmeas | 0.232 | 0.063 | 1.083 |
| Rpim | 0.068 | 0.018 | 0.315 |
| Total number of observations | 513794 | 10478 | 54790 |
| Number of reflections | 44169 | 881 | 4636 |
| <I/σ(I)> | 7.7 | 25.4 | 2.4 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 11.6 | 11.9 | 11.8 |
| CC(1/2) | 0.996 | 0.999 | 0.397 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 0.1 M Tris pH 8.5, 0.1 M MgCl2, 0.001 M NiCl2 and 22% PEG 3350 |
