6EEZ
Crystal Structure of the thiol-disulfide exchange protein alpha-DsbA2 from Wolbachia pipientis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.543 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.407, 104.796, 67.691 |
| Unit cell angles | 90.00, 96.48, 90.00 |
Refinement procedure
| Resolution | 19.768 - 2.250 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.213 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.051 | 0.265 |
| Rpim | 0.038 | |
| Number of reflections | 35424 | 3209 |
| <I/σ(I)> | 13.6 | 3.9 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100 mM Tris pH 7.5, 200 mM NaCl and 20% w/v PEG 3350 |
