6EEW
Crystal structure of Catharanthus roseus tryptophan decarboxylase in complex with L-tryptophan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-03-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.612, 69.607, 133.472 |
| Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
| Resolution | 133.182 - 2.050 |
| R-factor | 0.22074901198 |
| Rwork | 0.220 |
| R-free | 0.25561 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 133.190 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.800 | |
| Number of reflections | 256582 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.3 | 98.5 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.998 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.22M calcium chloride and 12% w/v PEG 3350 |
