6EEU

Structure of class II HMG-CoA reductase from Delftia acidovorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 3 2 1
Unit cell lengths	100.540, 100.540, 75.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.197 - 1.930
R-factor	0.1614
Rwork	0.160
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.794
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.200	1.990
High resolution limit [Å]	1.930	1.930
Number of reflections	33218
<I/σ(I)>	8.1
Completeness [%]	98.9
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	100 mM Bis-Tris, pH 5.5, 600 mM lithium sulfate, 18% PEG3350