6EEN
Crystal structure of a designer Pentatrico Peptide RNA binding protein, bound to a complex RNA target and featuring an infinite superhelix and microheterogeneity.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953737 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.347, 51.647, 51.972 |
| Unit cell angles | 118.12, 97.21, 96.00 |
Refinement procedure
| Resolution | 44.920 - 2.010 |
| R-factor | 0.1862 |
| Rwork | 0.183 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i9f |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.638 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.920 | 44.920 | 2.060 |
| High resolution limit [Å] | 2.010 | 8.980 | 2.010 |
| Rmerge | 0.055 | 0.036 | 0.305 |
| Rmeas | 0.074 | 0.049 | 0.413 |
| Rpim | 0.049 | 0.034 | 0.277 |
| Total number of observations | 50507 | 544 | 2884 |
| Number of reflections | 25127 | 285 | 1787 |
| <I/σ(I)> | 9 | 24.2 | 2.1 |
| Completeness [%] | 97.0 | 99.7 | 90.8 |
| Redundancy | 2 | 1.9 | 1.6 |
| CC(1/2) | 0.995 | 0.985 | 0.786 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Mg Acetate, 0.05 M MES pH 5.6, 20% MPD |
