6EEK
Crystal structure of apo Staphylcoccal nuclease variant Delta+PHS T62E/V66K, pH 7 at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2017-10-30 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 |
| Unit cell lengths | 71.795, 71.795, 56.804 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.180 - 2.200 |
| R-factor | 0.2564 |
| Rwork | 0.254 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.259 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.180 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.023 | 0.161 |
| Number of reflections | 8555 | 860 |
| <I/σ(I)> | 44.56 | 6.05 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 11 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM HEPES, 29% Jeffamine ED-2001 |
