6EEI
Crystal structure of Arabidopsis thaliana phenylacetaldehyde synthase in complex with L-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-08-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.064, 106.791, 115.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.537 - 1.990 |
| R-factor | 0.206283584534 |
| Rwork | 0.205 |
| R-free | 0.23985 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.704 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.900 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 66877 | 4433 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 10.2 | 10.3 |
| CC(1/2) | 0.997 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.16 M ammonium sulfate 0.8M HEPES:NaOH pH 7.5 and 20%w/v PEG 3350 |
