6EE0

Crystal Structure of SNX23 PX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9780
Spacegroup name	P 1 21 1
Unit cell lengths	46.783, 92.836, 47.039
Unit cell angles	90.00, 91.29, 90.00

Refinement procedure

Resolution	47.027 - 2.518
R-factor	0.1848
Rwork	0.178
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v14
RMSD bond length	0.010
RMSD bond angle	1.348
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.030	2.620
High resolution limit [Å]	2.518	2.518
Rmerge	0.062	0.684
Rpim	0.039	0.428
Number of reflections	13562	1488
<I/σ(I)>	8.8	1.3
Completeness [%]	99.0	96.7
Redundancy	3.4	3.4
CC(1/2)	0.998	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	50 mM Tris (pH 8.5), 0.2 M NaCl, 25% PEG 3350