6ED0
OhrA (Organic Hydroperoxide Resistance protein) mutant (C61S) in the "open conformation" from chromobacterium violaceum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-03-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 32 |
Unit cell lengths | 41.963, 41.963, 123.586 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 - 1.440 |
R-factor | 0.19239 |
Rwork | 0.191 |
R-free | 0.21321 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ecy |
RMSD bond length | 0.012 |
RMSD bond angle | 1.621 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.340 | 1.470 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmeas | 0.038 | 2.629 |
Rpim | 0.017 | 1.261 |
Number of reflections | 43151 | 1879 |
<I/σ(I)> | 25.5 | |
Completeness [%] | 97.9 | 84.1 |
Redundancy | 10.1 | 8.3 |
CC(1/2) | 1.000 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | purified ohr protein at 10mg/ml in 5mM Tris-Hcl, pH 7.4 & 50mM NaCl) Crystallisation conditions: Ammonium sulfate 200mM, Sodium acetate 100mM pH 4, PEG 2,000 30%w/v |