6EBN
Crystal structure of Psilocybe cubensis noncanonical aromatic amino acid decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 238.723, 143.570, 71.408 |
| Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
| Resolution | 71.785 - 1.966 |
| R-factor | 0.170814691565 |
| Rwork | 0.170 |
| R-free | 0.19739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hsj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.968 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.000 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 169024 | 7168 |
| <I/σ(I)> | 11.58 | |
| Completeness [%] | 99.1 | 85 |
| Redundancy | 6.8 | 6.4 |
| CC(1/2) | 0.998 | 0.469 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277.15 | 3.5 M Sodium Formate |
