6EBD

OhrB (Organic Hydroperoxide Resistance protein) mutant (C60A) from Chromobacterium violaceum, interacting with dihydrolipoamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-04-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 61
Unit cell lengths	87.675, 87.675, 179.609
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 2.610
R-factor	0.19633
Rwork	0.194
R-free	0.23214
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eb4
RMSD bond length	0.012
RMSD bond angle	1.830
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.840	2.730
High resolution limit [Å]	2.610	2.610
Rmeas	0.343
Rpim	0.105	1.526
Number of reflections	23763	60434
<I/σ(I)>	10.82
Completeness [%]	99.9
Redundancy	20.8
CC(1/2)	1.000	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	Purified Ohr_C60A protein 10mg/ml in 5mM Tris-Hcl pH 7.4 Crystallisation conditions: Magnesium Clhoride 200mM, BIS-TRIS 100mM pH 5.5, PEG 3,350 25%w/v crystal was soaked for 10min in Lipoamide 5mM, solved in 5% ethanol and Crystallisation condition