6EBC
OhrB (Organic Hydroperoxide Resistance protein) wild type from Chromobacterium violaceum and reduced by DTT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 273.15 |
Detector technology | PIXEL |
Collection date | 2016-06-08 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.458660 |
Spacegroup name | P 61 |
Unit cell lengths | 88.160, 88.160, 169.916 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 - 1.870 |
R-factor | 0.19415 |
Rwork | 0.193 |
R-free | 0.21446 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6eb4 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.813 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.490 | 1.910 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmeas | 0.114 | 2.798 |
Rpim | 0.038 | 1.275 |
Number of reflections | 61621 | 3977 |
<I/σ(I)> | 19 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 17.2 | 9.3 |
CC(1/2) | 0.999 | 0.356 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | Purified protein 10mg/ml in 5mM Tris-Hcl, pH 7.4 and DTT 10mM Crystallization solution: Magnesium Chloride 200mM, BIS-TRIS 100mM pH 5.5, PEG 3,350 25%w/v Then, Soaked with Cs + DTT 50mM |