6EB7

YycF homologue (SP1227) Receiver Domain Activated by BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-06
Detector	MAR CCD 130 mm
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	74.563, 93.324, 37.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 1.580
R-factor	0.196
Rwork	0.195
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.210	1.636
High resolution limit [Å]	1.580	1.580
Rpim	0.061	0.614
Number of reflections	17717	1742
<I/σ(I)>	11.38	1.09
Completeness [%]	96.4	95.65
Redundancy	2	2
CC(1/2)	0.995	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	36% v/v PEG 300, 5% w/v PEG 1,000 100 mM Sodium Acetate pH 5.5