6EAJ
CRYSTAL STRUCTURE OF HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION GLYCOPROTEIN INHIBITOR ESCAPE VARIANT T400A STABILIZED IN THE PREFUSION STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-06-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.181 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 168.190, 168.190, 168.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.060 - 2.851 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ea4 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.060 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.141 | 0.974 |
| Rmeas | 0.159 | 1.094 |
| Rpim | 0.072 | 0.489 |
| Total number of observations | 92996 | |
| Number of reflections | 19529 | 2775 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 4.8 | 4.9 |
| CC(1/2) | 0.993 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 1.50M K/Na tartrate, 0.2M LiSO4, 0.1M CHES pH 9.5 |
