6EAF
CRYSTAL STRUCTURE OF HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION GLYCOPROTEIN INHIBITOR ESCAPE VARIANT G143S STABILIZED IN THE PREFUSION STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 167.249, 167.249, 173.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.880 - 3.000 |
| R-factor | 0.1991 |
| Rwork | 0.198 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ea4 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.194 | 1.265 |
| Rmeas | 0.210 | 1.363 |
| Rpim | 0.079 | 0.501 |
| Total number of observations | 335941 | |
| Number of reflections | 49667 | 4510 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.992 | 0.581 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 1.60M K/Na tartrate, 0.2M LiSO4, 0.1M CHES pH 9.5 |
