6E9Q

The crystal structure of bovine ultralong antibody BOV-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-13
Detector	RAYONIX MX-300
Wavelength(s)	0.978560
Spacegroup name	P 21 21 21
Unit cell lengths	64.044, 72.687, 452.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.693 - 3.400
R-factor	0.2567
Rwork	0.254
R-free	0.31070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.833	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.109	0.310
Number of reflections	30383
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	18% PEG 8000, 0.1 M HEPES pH 7.5