6E9I

The crystal structure of bovine ultralong antibody BOV-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-08
Detector	RAYONIX MX-300
Wavelength(s)	0.978560
Spacegroup name	P 32 2 1
Unit cell lengths	148.547, 148.547, 165.443
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.867 - 2.500
R-factor	0.2044
Rwork	0.202
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.650	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.054	0.456
Number of reflections	66205
<I/σ(I)>	20.3
Completeness [%]	90.1
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	30% PEG 1000, 0.1 M sodium malonate pH 8.0, 0.1 M Tris-HCl pH 8.0