6E9B
Bacteroides ovatus mixed-linkage glucan utilization locus (MLGUL) SGBP-B in complex with mixed-linkage heptasaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-08 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 156.292, 242.207, 75.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.330 - 3.150 |
R-factor | 0.224 |
Rwork | 0.221 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e57 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.340 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 50094 | 7894 |
<I/σ(I)> | 15.6 | 1.73 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.42 | 7.44 |
CC(1/2) | 0.999 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 296 | 0.2M lithium sulfate, 0.1M phosphate-citrate, 20% (w/v) PEG1000 |