6E94

Crystal Structure of ZBTB38 C-terminal Zinc Fingers 6-9 K1055R in complex with methylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	277.15
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	70.804, 77.270, 103.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.402 - 1.594
R-factor	0.1963
Rwork	0.194
R-free	0.21830
RMSD bond length	0.007
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.800	1.620
High resolution limit [Å]	1.590	1.590
Number of reflections	37856
<I/σ(I)>	9.4
Completeness [%]	99.3
Redundancy	12.9
CC(1/2)	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277.15	0.25M ammonium Chloride MES (pH:6.0) 19% PEG 6000