6E84
Crystal structure of the Corn aptamer in complex with TO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 128.611, 128.611, 40.843 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.346 - 2.901 |
| R-factor | 0.2401 |
| Rwork | 0.234 |
| R-free | 0.29310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e81 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.451 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.350 | 38.350 | 2.920 |
| High resolution limit [Å] | 2.850 | 12.750 | 2.850 |
| Rmerge | 0.086 | 0.028 | 1.049 |
| Rmeas | 0.088 | 0.028 | 1.079 |
| Rpim | 0.020 | 0.007 | 0.250 |
| Total number of observations | 58519 | ||
| Number of reflections | 3104 | 42 | 244 |
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 100.0 | 98 | 100 |
| Redundancy | 18.9 | 15.5 | 18 |
| CC(1/2) | 1.000 | 1.000 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 20 % PEG 4000, 5% PEG 400, 10% glycerol, 0.2 M ammonium acetate pH 6.7, 0.1 M sodium citrate pH 5.6, 0.5 mM thiazole orange |
