6E80
Crystal structure of the Corn aptamer in unliganded state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-10 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 1.10503 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 93.012, 93.012, 66.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.506 - 2.901 |
| R-factor | 0.2575 |
| Rwork | 0.252 |
| R-free | 0.30710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e81 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.292 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.147 | 0.140 | 0.455 |
| Rmeas | 0.184 | 0.173 | 0.644 |
| Rpim | 0.109 | 0.100 | 0.455 |
| Total number of observations | 8472 | ||
| Number of reflections | 3961 | 219 | 134 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 89.2 | 89.8 | 63.5 |
| Redundancy | 2.1 | 2.4 | 1.3 |
| CC(1/2) | 0.938 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 20% PEG 4000, 0.2 M ammonium acetate pH 6.7, sodium citrate pH 5.6 |
