6E7O

Crystal structure of deglycosylated human EPDR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	87.805, 97.482, 189.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.687 - 3.000
R-factor	0.2557
Rwork	0.255
R-free	0.27810
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3211))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.700	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	17638
<I/σ(I)>	15.7	1.9
Completeness [%]	99.9
Redundancy	8.3	8.4
CC(1/2)	0.998	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M lithium sulfate, 0.1 M sodium acetate pH 4.5, 30% PEG8000, 4% propanediol