6E6Z

Structure of Wild Type Human Transthyretin in Complex with Tafamidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	41.240, 84.260, 63.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.430 - 1.750
R-factor	0.195
Rwork	0.192
R-free	0.22300
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.430	63.430	1.790
High resolution limit [Å]	1.750	7.820	1.750
Rmerge	0.055	0.041	0.714
Rmeas	0.057	0.043	0.744
Number of reflections	23032	312	1681
<I/σ(I)>	24.57	52.9	3.64
Completeness [%]	99.8	100	98.5
Redundancy	12.816	11.417	12.434
CC(1/2)	0.999	0.999	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	20% (W/V) PEG-1000, 0.1M Imidazole, 0.2M Calcium Acetate