6E6O
Pheromone from Euplotes raikovi, Er-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.6192 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.440, 22.560, 23.010 |
| Unit cell angles | 90.00, 110.57, 90.00 |
Refinement procedure
| Resolution | 25.020 - 0.700 |
| R-factor | 0.16632 |
| Rwork | 0.165 |
| R-free | 0.18445 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.682 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.020 | 0.725 |
| High resolution limit [Å] | 0.700 | 0.700 |
| Rmerge | 0.095 | 1.630 |
| Number of reflections | 39777 | |
| <I/σ(I)> | 9.41 | |
| Completeness [%] | 97.0 | |
| Redundancy | 12.3 | |
| CC(1/2) | 0.999 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 290 | 0.1 M to 0.2 M (NH4)2SO4, 0.05 M Na Citrate pH 3.5 and 6% (v/v) ethanol, using 5-ul protein drops and 5 ul of reservoir solution |
