6E6N
Pheromone from Euplotes raikovi, Er-13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.6198 |
| Spacegroup name | P 43 |
| Unit cell lengths | 39.116, 39.116, 19.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.120 - 1.363 |
| R-factor | 0.15506 |
| Rwork | 0.152 |
| R-free | 0.18030 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.846 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.120 | 1.411 |
| High resolution limit [Å] | 1.360 | 1.363 |
| Rmerge | 0.052 | 0.893 |
| Number of reflections | 85988 | |
| <I/σ(I)> | 9.41 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 | |
| CC(1/2) | 1.000 | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 0.1 M to 0.2 M (NH4)2SO4, 0.05 M Na Citrate pH 3.5 and 6% (v/v) ethanol, using 5-ul protein drops and 5 ul of reservoir solution |
