6E63
Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody TB31F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 51.994, 120.907, 177.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.899 - 2.600 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.23980 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.760 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 35359 | 3678 |
| <I/σ(I)> | 8.9 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.2 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris, pH 6.0, 22% (w/v) PEG 3350, 0.2 M NaCl |
