6E4P

Structure of the T. brucei RRM domain in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-23
Detector	ADSC QUANTUM 210r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	49.123, 126.297, 49.203
Unit cell angles	90.00, 118.57, 90.00

Refinement procedure

Resolution	35.661 - 1.949
R-factor	0.183
Rwork	0.181
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e4n
RMSD bond length	0.005
RMSD bond angle	0.848
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.661	2.000
High resolution limit [Å]	1.949	1.949
Rpim	0.040	0.280
Number of reflections	38345
<I/σ(I)>	17.8
Completeness [%]	99.8
Redundancy	3.8	2.5
CC(1/2)	0.998	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	citrate as precipitant