6E4N

Structure of the T. brucei TbRGG2 RRM domain: apo R3 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	59.871, 59.871, 57.571
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.264 - 1.801
R-factor	0.1533
Rwork	0.152
R-free	0.18750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2m8d
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.264	1.990
High resolution limit [Å]	1.800	1.800
Rpim	0.040	0.161
Number of reflections	7045
<I/σ(I)>	11.3
Completeness [%]	99.0	91.3
Redundancy	1.7	1.7
CC(1/2)	0.994	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.4 M citrate, 0.1 M Bis-Tris pH 7 or HEPES